ISSN:
1432-0827
Keywords:
Interleukin-1
;
Indomethacin
;
Prostaglandin E2
;
Bone formation
;
Intramedullary infusion
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
,
Physics
Notes:
Abstract Interleukin-1 (IL-1) is known to have a potent bone-resorbing activity in vitro, however, results from in vivo studies are conflicting. We performed experiments with the continuous infusion of recombinant IL-1α directly into the femoral bone marrow of rats for 2 weeks and examined its effects on bone by soft X-ray photographs, bone mineral assessment, and histological examination. Infusion of IL-1α at doses greater than 1 μg/ml (0.6 ng/hour) caused sclerosis around the infusion site on soft X-ray photographs, and the bone mineral content of IL-1α infused femora was increased significantly. Histologically, extensive periosteal bone formation was observed around the infusion site and small mononuclear cells replaced the normal fat tissue. Infusion of prostaglandin E2 alone produced intramedullary bone formation more extensively. Simultaneous infusion of IL-1α and indomethacin (10-3 M; 179 ng/hour) inhibited the increase of bone mineral content (BMC) induced by IL-1α. Thus, the chronic intramedullary administration of IL-1α stimulates bone formation, especially in the periosteum, and increases BMC in intact rats.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00297184
