ISSN:
1432-1424
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Using the short-circuit current as a measure of the electrogenic chloride transport in the salt glands ofLimonium, the effects of various inhibitors, of light-dark changes and of oxygen removal have been studied during steady-state pumping. The results are consistent with the hypothesis that ATP is the energy source for the chloride pump in this system. When microsomes from salt-loaded tissue are tested for ATPase activity, a substantial fraction of this is found to be chloride-stimulated. In uninduced tissue the Cl-ATPase activity is very much lower, and the induction by salt-loading can be blocked by puromycin. The parallels with Cl-pumping in this tissue are close enough to assume that the Cl-ATPase activity is that of the pump itself; the way is therefore open to study the pumpin vitro.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01869996
