ISSN:
1432-1424
Keywords:
transepithelial Na transport
;
apical Na permeability
;
Na-channel density
;
aldosterone
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Chemistry and Pharmacology
Notes:
Summary Near-instantaneous current-voltage relationships and shot-noise analysis of amiloride-induced current fluctuations were used to estimate apical membrane permeability to Na (P Na), intraepithelial Na activity (Na c ), single-channel Na currents (i) and the number of open (conducting) apical Na channels (N0), in the urinary bladder of the toad (Bufo marinus). To facilitate voltageclamping of the apical membrane, the serosal plasma membranes were depolarized by substitution of a high KCl (85mm) sucrose (50mm) medium for the conventional Na-Ringer's solution on the serosal side. Aldosterone (5×10−7 m, serosal side only) elicited proportionate increases in the Na-specific current (I Na and inP Na, with no significant change in the dependence ofP Na on mucosal Na (Na o ).P Na and the control ofP Na by aldosterone were substrate-dependent: In substrate-depleted bladders, pretreatment with aldosterone markedly augmented the response to pyruvate (7.5×10−3 m) which evoked coordinate and equivalent increases inI Na andP Na. The aldosterone-dependent increase inP Na was a result of an equivalent increase in the area density of conducting apical Na channels. The computed single-channel current did not change. We propose that, following aldosterone-induced protein synthesis, there is a reversible metabolically-dependent recruitment of preexisting Na channels from a reservoir of electrically undetectable channels. The results do not exclude the possibility of a complementary induction of Na-channel synthesis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01870771
