ISSN:
1432-1432
Keywords:
Prebiotic
;
Polymerization
;
Deoxythymidine oligonucleotides
;
Deoxythymidine 5′-triphosphate
;
Deoxythymidine 5′-monophosphate
;
4-amino-5-imidazolecarboxamide
;
Cyanamide
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary When an aqueous solution (pH 7.0) of3H deoxythymidine 5′-triphosphate, deoxythymidine 5′-phosphate, 4-amino-5-imidazolecarboxamide, cyanamide and ammonium chloride was dried and heated at 60°C for 18 h, oligomers were obtained in a yield of approximately 80%. After the chemical degradation of any pyrophosphate bonds present in these oligomers, linear polynucleotides of up to 7–8 units in length were isolated by DEAE cellulose column chromatography and identified by enzymatic digestion procedures. The di- and trinucleotide fractions were degraded 87% and 100% by snake venom phosphodiesterase and 39% and 9% by spleen phosphodiesterase. This synthesis of deoxythymidine oligonucleotides was conducted under potentially prebiotic conditions and may offer a possible method for the synthesis of deoxyoligonucleotides on the primitive Earth.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01764595
