ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The stability of plasmids inLactobacillus plantarum was investigated by extended incubation of bacterial cells in the presence of different carbohydrates. Strain caTC2, carrying a plasmid-encoded chloramphenicol-resistant (Cmr) phenotype, was grown overnight (16–18 h) in MRS, MRS-L, and MRS-M broths containing 2% glucose, lactose, and maltose respectively at 30°C. The cultures were subsequently held at 30°C and room temperature (21±1°C) for an extended period (7 days). The total viable cell counts were assayed on MRS agar plates and tested for sensitivity to 30 μg chloramphenicol/ml by replica plating. The plasmid profiles of the chloramphenicol-sensitive strains showed that there was a loss of the 8.5-kb plasmid, but not the 10.6 or 6.5 kb plasmids. Concomitant loss of the chloramphenicol resistance phenotype and plasmid at high frequency, particularly by using MRS-L at 21°C method, suggests that this would be a simple and efficient method for curing selected plasmids in lactobacilli.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01570722
