ISSN:
1432-0991
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
,
Medicine
Notes:
Abstract The location of extracytoplasmic acid phosphatase in haploid, diploid, and polyploid cells ofSaccharomyces cerevisiae was examined by cytochemical electron microscopy. In all cases, in the presence of lead nitrate and low concentrations of glycerophosphate, the reaction product (lead phosphate) was restricted to the periplasmic space. With higher substrate concentrations (which are typical of those previously employed by others) precipitates also appeared on the cell wall surface of some specimens; a result previously reported by three other laboratories. The surface deposits are deemed artifacts from incomplete lead capture under high enzymatic rates of orthophosphate generation. A model system that supports this is presented.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF01566599
