ISSN:
1279-8509
Keywords:
Hemoglobin Moabit
;
Electrophoresis
;
Globin chains
;
Chromatography
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Abstract An abnormal hemoglobin fraction was detected on high performance liquid chromatography profile performed for the measurement of glycated hemoglobin in a 55-year-old caucasian patient. The structural and functional studies were performed by standard techniques. Separation of hemoglobins by alkaline electrophoresis and by IEF revealed a slightly more rapid fraction than does Hb S. By acid electrophoresis, no abnormal Hb fraction could be observed. Separation of globin chains by electrophoresis demonstrated an alpha-chain variant and by chromatography, a fraction which eluted between ß and gamma globin chains. Tryptic digests and amino acid analysis have demonstrated a previously described substitution of LeuArg alpha 86(F7).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s00282-997-0137-x