ISSN:
1610-7403
Keywords:
Agrobacterium tumefaciens
;
lombardy polar
;
Populus nigra L. var.italica Koehne
;
shoot regeneration
;
transformation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
Notes:
Abstract Genetically transformed lombardy poplar (Populus nigra L. var.italica Koehne) plants were regenerated by co-cultivation of stem segments withAgrobacterium tumefaciens strain LBA4404 that harbored a binary vector (pBI121) which included genes for β-glucuronidase (GUS) and neomycin phosphotransferase. Successful transformation was confirmed by the ability of stem segments to produce calli in the presence of kanamycin, histochemical and fluorometric assays of GUS activity in plant tissues, and Southern blot analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02348333
