ISSN:
1432-8798
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Medizin
Notizen:
Summary The sequential changes in FL cells infected with para-influenza 3 virus under conditions in which persistent infection develops were studied by light microscopy, specific immuno-fluorescence and electron microscopy. Hemadsorption in this system was also examined by electron microscopy. The giant multinucleated cell, the characteristic initial cytopathic effect, was shown to be truly syncytial. Specific immunofluorescence appeared in syncytia in the nuclei and at the nuclear membrane; later when the giant cells had broken down and proliferating pleomorphic, spindle-shaped and rounded cells appeared, fluorescence was most pronounced at the plasma membranes. Virus particles which arise from the plasma membranes were first seen along the plasma membrane of otherwise unchanged cells just outside the syncytia. There was extensive virus release at the borders of spindle-shaped cells and in the region of virus release the cytoplasm was finely filamentous. The virus particles ranged in size from 100 to 160 mμ. They were seen to have a limiting membrane with an inner structure consisting of several dense, round profiles. There was also an outer amorphous, ill-defined layer. Definite changes in the ultrastructure of the nucleus were infrequent but small granules in the nuclear matrix with diameters of 11 to 17 mμ were of special interest. Both virus-and cyto-hemadsorption were seen but the pattern differed from that seen with influenza virus.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF01241303
