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An improvement in the plaque assay of rabies virus in chick embryo cells (1971)

Yoshino, K. ; Morishima, T.

Springer

Archives of virology 34 (1971), S. 40-50

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ISSN: 1432-8798

Source: Springer Online Journal Archives 1860-2000

Topics: Medicine

Notes: Summary The faint plaques formed with HEP Flury strain of rabies virus in chick embryo cells by the original method were thought ascribable to acid production from virus-infected cells coupled by the weak buffering action of the agar overlay medium. When the overlay medium contained appropriate concentrations of alkalies, clear plaques could be observed. The optimal conditions were (i) incorporation of 0.02% NaOH, 0.0025m Tris-HCl buffer of pH 8.2 and 2% calf serum in the base overlay medium, and (ii) neutral red staining after 7 days' incubation at 35

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01250244

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