ISSN:
1434-4475
Keywords:
Enzymic synthesis in biphasic systems
;
Pepsin-catalyzed peptide bond formation
;
Peptide synthesis
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Abstract The usefulness of biphasic aqueous-organic solvent systems for pepsin-catalyzed synthesis of model peptidesZ-X-Phe-Phe-OMe (X=Ala, Gln, Leu) has been demonstrated by coupling the correspondingZ-X-Phe-OH with H-Phe-OMe. The influence of various organic solvents on pepsin activity was examined. Some examples are given for the influence of nucleophile and enzyme concentration, bufferpH and organic solvent portion on product yield. Tetrachloromethane and mixtures of ethyl acetate/n-hexane proved to be especially useful allowing syntheses in good yields and within comparatively short reaction times of 2–6 hours.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00798612
