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Quantitative analysis of calcium gradients and activity in growing pollen tubes ofLilium longiflorum (1987)

Nobiling, R. ; Reiss, H. -D.

Springer

Protoplasma 139 (1987), S. 20-24

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ISSN: 1615-6102

Keywords: Calcium activity ; Calcium gradient ; Lilium ; Polar growth ; Pollen tube ; Quin-2 ; Microfluorimeter

Source: Springer Online Journal Archives 1860-2000

Topics: Biology

Notes: Summary Pollen tubes ofLilium longiflorum were loaded with quin-2 to determine the cytoplasmic free calcium. Quin-2-fluorescence was detected at 500 nm with alternating excitation at 340 nm and 360 nm. The calcium2+-concentration was obtained using the intensity ratio R=I340/I360. The analysis exhibits a [Ca2+] of nearly 10−7mol·l−1 in the tip region and about 2·10−8 mol·l−1at the tube base, near the pollen grain. The data give evidence for the existence of a continuous gradient of free calcium within growing pollen tubes of various length.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF01417531

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