ISSN:
1612-1112
Keywords:
Gas chromatography
;
Capillary columns
;
Steroid profiles
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Steroid conjugates are hydrolysed enzymatically using β-glucuronidase after extraction from urine using a solid phase extraction cartridge. After hydrolysis the free steroids are removed from the matrix, again utilising solid phase extraction. Derivatisation of the free hydroxyl groups using Hydrox-Sil AQ produces the respective TMS ethers which are extracted into hexane, in which solvent they are stable for many days. Capillary GC analysis with flame ionisation detection produces a profile of the steroids present in the sample. This technique is suitable for following changes in the urinary excretion profiles of patients undergoing investigation for a variety of steroid production-related diseases.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02327654
