ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Buffer composition effect
;
Agarose
;
Albumin
;
Ionic adsorption
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The adsorption of albumin on a commercial, high quality agarose (Sepharose® 6B) was studied in different buffers of low pH (4.00) and low molarity (9–11 mM). Eluents containing 0.025% albumin were introduced onto small gel columns until saturation and the adsorption capacity of the gel in different buffers calculated. Adsorption was high in solutions of monobasic acids. Although cation-exchange interactions between the protein and the gel matrix were found to be influenced essentially by the sodium ion concentration or ionic strength of the buffer, adsorption in cirate buffers is weak compared to that in formate, acetate or propionate buffers of about the same ionic strength (I=0.01).
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02278590
