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Analysis of n-3 fatty acids in fish oils by high-performance liquid chromatography (1993)

Teng, J. I. ; Made Gowda, N. M.

Springer

Chromatographia 35 (1993), S. 627-630

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ISSN: 1612-1112

Keywords: Column liquid chromatography ; Fatty acid analysis ; Fish oils

Source: Springer Online Journal Archives 1860-2000

Topics: Chemistry and Pharmacology

Notes: Summary A chromatographic method has been developed for the determination of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) in fish oil dietary supplements containing triglycerides rich in (n−3) polyunsaturated fatty acids (PUFAs). Following the ester saponification, free fatty acids were resolved by TLC into two fractions: i) a more mobile group of saturated and monounsaturated fatty acids and ii) a less mobile group containing PUFAs. The PUFA fraction was further analyzed by HPLC to determine the levels of EPA and DHA in the fish oil sample.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1007/BF02267927

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