ISSN:
1612-1112
Keywords:
Column liquid chromatography
;
Mixed-bed stationary phase
;
Anion and cation exchange resins
;
Retention mechanism of anions and cations
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary The retention mechanism of anions and cations on a mixed-bed column packed with a mixture of strong anion and cation exchange resins has been studied. Organic acids and inorganic anions have showed the same elution behaviour on the mixed-bed column as on a single anion exchange column. In contrast, different elution behaviours for cations have been observed between the mixed-bed column and a single cation exchange column. On the mixed-bed column, cations would be eluted by a mixed retention mechanism. The primary retention mechanism is ion exchange with eluent cation (H+) for all cations. The ion exchange mechanism on the sites occupied by anion exchange groups makes a greater contribution for monovalent cations. A partition mechanism between the stationary phase and mobile phase contributes to the separation of Mg2+ and Ca2+ by forming neutral compounds.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02290265
