ISSN:
1612-1112
Keywords:
Gas chromatography
;
HBr-treated capillary column
;
Methylmercury chloride
;
Halide exchange
;
ECD
Source:
Springer Online Journal Archives 1860-2000
Topics:
Chemistry and Pharmacology
Notes:
Summary Gas chromatography with electron capture detection (GC-ECD) for the analysis of methylmercury choloride (MMC) using a packed column and a capillary column has been investigated. The columns were 2% silicone OV-227 Uniport HP glass column and a DB-17 capillary column, each pretreated by about ten injections of HBr-methanol solution. MMC was separated as a sharp peak by the HBr-teated column and determined directly by ECD without derivatisation. The mass spectrum of MMC indicated that halide exchange from chloride to bromide proceeded during separation. The minimum detectable concentrations were approximately 5 ng mL−1 on the packed column, and 2 ng mL−1 on the capillary. Calibration curves showed good linearity between 5–200 ng mL−1 for the packed column, and between 2–200 ng mL−1 for the capillary. Relative standard deviations of peak areas were 0.95% for the packed column and 0.43% for the capillary at the level of 100 ng mL−1 in both cases. The column treatment technique was applicable to determination of methylmercury in fish samples.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF02466315
