ISSN:
1612-1112
Schlagwort(e):
Supercritical fluid chromatography
;
Semi-preparative chromatography
;
Peak compression effect
;
Dihydropyridine drug
Quelle:
Springer Online Journal Archives 1860-2000
Thema:
Chemie und Pharmazie
Notizen:
Summary Peak compression of a dihydropyridine drug, clevidipine, is obtained in both analytical and semi-preparative scale supercritical fluid chromatography, resulting in extremely high apparent efficiencies. The observed effect, when utilising a carbon dioxide/2-propanol mobile phase with a bare silica stationary phase, is achieved when the retention of the clevidipine peak is controlled to coalesce with a system peak, generated as a result of having water in the sample. Apparent efficiencies of 350,000 and 170,000 plates meter−1 were obtained when 0.25 and 0.5 mg, respectively, are directly injected to a 200×4.6 mm ID 5 μm Hypersil silica packed column. The effect was extended to a semi-preparative system where apparent efficiencies in the region of 2,000,000 plates meter−1 were observed when 0.3 mg of a clevidipine sample containing 80% water was injected to a 250×10 mm I.D. column containing 5-μm Hypersil silica particles.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1007/BF02467565
