Electronic Resource
[S.l.]
:
International Union of Crystallography (IUCr)
Acta crystallographica
32 (1976), S. 67-74
ISSN:
1600-5724
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The neutron small-angle scattering of hen egg-white lysozyme chloride in solution has been determined. The scattering density of the solvent and accordingly its contrast with the dissolved particles has been varied by changing the H2O/D2O ratio of the solvent. The zero-angle scattering was derived and it was shown that the square root of the zero-angle intensity is proportional to the contrast. The experimental value of the radius of gyration is R = 13.8 Å, in good agreement with the same quantity calculated on the basis of the crystal structure model of tetragonal lysozyme. The three basic scattering functions were derived from the experimental data and compared with the corresponding scattering pattern calculated from the coordinates of the X-ray structure. The results do not suggest a deviation of the conformation and structure of lysozyme in solution from the crystal structure. The spherical average of the overall dimensions has been separated from the internal structure. The influence of higher multipoles of the shape of the lysozyme molecule on the scattering curve is discussed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0567739476000132
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