ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
The NAD+-linked glycerol dehydrogenase from Bacillus stearothermophilus is a member of the so-called `iron- containing' class of polyol dehydrogenases. This enzyme has been crystallized in three different forms in the presence of a range of divalent cations and glycerol or NAD+ using the hanging-drop method of vapour diffusion with ammonium sulfate as the precipitant. X-ray photographs have established that the crystals grown in the presence of zinc and glycerol (form A) most likely belong to space group I4122 with cell parameters a = b = 102 and c = 728 Å. The crystals grown with zlnc and NAD+ (form B) belong to the tetragonal system and probably belong to the space group P4212 with cell parameters a = b = 150 and c = 220 Å. The crystals grown with lead and glycerol (form C) belong to a primitive orthorhombic system with cell parameters a = 127, b = 178 and c = 173 Å. Experiments using the synchrotron radiation source at the DRAL Daresbury laboratory have shown all three crystal types diffract to at least 3 Å resolution. Elucidation of the three-dimensional structure of this enzyme will provide a structural framework for this class of polyol dehydrogenases, which are not represented in the database at present, and enable comparisons to be made with enzymes belonging to the other classes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S0907444994013533
