ISSN:
1399-0047
Source:
Crystallography Journals Online : IUCR Backfile Archive 1948-2001
Topics:
Chemistry and Pharmacology
,
Geosciences
,
Physics
Notes:
Chitosanase, an enzyme which hydrolyzes chitosan, isolated from Bacillus circulans MH-K1, was crystallized by a vapor-diffusion procedure at 293 K using ammonium sulfate as a precipitant. Rod-shaped colorless crystals, which grew to 0.05 × 0.15 × 1.2 mm within a week, belong to the orthorhombic system and the space group P2221 or P21212 with unit-cell dimensions of a = 43.3, b = 57.7, and c = 128.0 Å. The asymmetric unit is thought to contain one chitosanase molecule (29 024 Da). The crystals diffract X-rays to at least 2.3 Å resolution and are suitable for high-resolution X-ray structure analysis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1107/S090744499500268X
