ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
In cell culture medium containing serum proteins, a layer with a great role inostelblast-like cell’s growth formed on a HAp ceramics by a coprecipitation with a deposition of a bone-like apatite and an adsorption of serum proteins. The adsorption of proteins is influential in the apatite deposition and the cell adhesion. A component of serum albumin is forming 〈 80% of total serum proteins, therefore we focused on the serum albumin. The serum albumin was removed fromfetal bovine serum by using an albumin removal kit, and then the albumin free cell culture medium was prepared. The bone-like apatite layer was quickly formed on the HAp ceramics in the albumin free medium, however the high cell adhesion property was not confirmed. As a result of investigation of an initial cell adhesion, the initial cell adhesion property of the bone-like apatite layer was not excellent even though the layer contained the serum proteins. Moreover, the layer without serum proteins showed a meager cell adhesion property in every time of the cultivation.Therefore, it was revealed that the adsorption of the serum proteins only on the surface was not enough to the improvement of the cell growth. And a few days cultivation was required for an appearance of the remarkable effect on the cell growth on the layer formed by a coprecipitation reaction about the bone-like deposition apatite and the serum proteins adsorption
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/50/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.309-311.283.pdf
