ISSN:
1013-9826
Source:
Scientific.Net: Materials Science & Technology / Trans Tech Publications Archiv 1984-2008
Topics:
Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
Notes:
Recently, nanomaterials have received considerable attention because of their potentialapplications in the biomedical field. In the present study, we investigated the effects of nano-sizedcalcium metaphosphate (CMP) particles (50 nm) compared with micro-sized CMP particles (200-500nm and 10 μm) on the proliferation and osteoblastic differentiation of human bone marrow stem cells(BMSCs). BMSCs were challenged with CMP particles with different sizes for 3, 5, and 7 days. Ananalysis of the proliferation revealed that the nano-sized CMP particles (50 nm) stimulated theproliferation of BMSCs up to 27.79% compared to the untreated control. This stimulatory effect ofthe nano-sized CMP particle was dose-dependent. CMP particles appeared to adhere on the surface ofBMSCs but this did not cause distinguishable morphological changes. Moreover, all CMP particles(50 nm to 10 μm) were capable of stimulating an osteoblastic differentiation of BMSCs as accessedby alkaline phosphatase (ALP) and von Kossa stainings. Further molecular analysis revealed that allthe CMP particles induced an expression of osteoblast-related genes such as osteocalcin (OC) andcollagen I (Col I). Taken together, our data demonstrate that nano-sized CMP particles have thepotential to stimulate the proliferation and osteoblastic differentiation of BMSCs
Type of Medium:
Electronic Resource
URL:
http://www.tib-hannover.de/fulltexts/2011/0528/01/56/transtech_doi~10.4028%252Fwww.scientific.net%252FKEM.361-363.1177.pdf
