ISSN:
1365-2230
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Nickel antigen (63Ni) binding by serum components and uptake by mononuclear cells were studied in 16 healthy and nickel-sensitive subjects, respectively. Ftaorography of serum proteins revealed five main 63Ni-bindmg tractions with molecular weights of 60–70 kd, 50 kd, 41–42 kd, 38–39 kd, and 24 kd. Only quantitative differences were seen between individuals.Autoradiography studies of cells incubated 5 h with nickel revealed 63Ni uptake by adherent monocytes. T-cells did no bind 63Ni in this early phase. 63Ni uptake In mononuclear cells was followed during a 7-day nickel-induced blast transformation. The uptake peak was reached within 1 h and there was another peak of 63Ni hinding on Day 7 of the culture, when the nickel blast transformation was in an exponential growth phase. Cells collected at this stage were analysed in SDS-page electrophoresis followed by fluorography. This revealed two 63Ni-binding protein fractions with molecular weights of 27 kd and 11 kd, respectively, in both nickel-sensitive and healthy subjects. Nickel-binding proteins obtained in serum and cells did not reveal differences between healthy and nickel-sensitive subjects in the handling of nickel antigen.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2230.1987.tb01916.x
