Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
FEMS microbiology letters
34 (1986), S. 0
ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract Escherichia coli cells were labeled with [32P]orthophosphate under anaerobic conditions using nitrate as electron acceptor. By 2-dimensional gel electrophoresis, 20 protein species were found to be radioactively labeled; their isoelectric points and molecular masses were determined. Treating the labeled extract with alkaline phosphatase revealed evidence for the presence of 9 proteins modified by phosphorylation. Treatment with snake venom phosphodiesterase also indicated that 2 protein species in E. coli were modified by phosphate-containing compounds which were bound to the protein by a phosphodiester bond.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6968.1986.tb01410.x
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