ISSN:
1574-6941
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
Abstract The colonization of biofilms of a benzoate-degrading Gram-positive water bacterium, strain B4, by a pathogenic Escherichia coli was studied in a continuous flow reactor. E. coli added to a fixed bed reactor colonized by B4, was able to grow in the biofilms and subsequently re-enter the free water phase in high numbers. Mixed biofilms of strain B4 and E. coli were also grown on glass slides for detailed examination of the spatial order of the mixed population biofilm. Individual cells as well as microcolonies of E. coli were detected in the biofilms by hybridization with a fluorescently labeled 23S rRNA-targeted oligonucleotide probe. The spatial distribution of E. coli could be analyzed in all layers of even thick biofilms.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1574-6941.1994.tb00063.x