ISSN:
1540-8191
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Treatment of glutaraldehyde-fixed pericardium with L-glutamic acid and storage in bacteriostatic preservatives (paraben) stably antagonizes free, reactive aldehyde groups within the fixed bioprosthetic heart valve tissue. In 63-day subcutaneous implants in rats, the calcification rate of this treatment (13.3 ± 2 mg calcium/g wt tissue) was markedly reduced as compared to conventionally treated tissue (169 ± 24 mg/g; p 〈 0.05). To test the influence of tissue released toxic aldehdyes on spontaneous endothelial cell ingrowth in vivo, vascular grafts (8-cm long, 6-mm diameter) from fixed pericardium treated with L-glutamic acid were interposed into the carotid arteries in ten sheep. They were compared to grafts from conventionally treated pericardium implanted at the contralateral side. Following 3 months of implantation, planimetry revealed 49%± 20% of the surface of conventionally preserved pericardium to be covered with red thrombus, but only 12%± 5% in L-glutamic acid treated pericardium (p 〈 0.05). The ultrastructural findings of a closed endothelial cell layer on the graft surface reveals the new technique to be a promising approach towards increased biocompatibility of aldehyde-fixed bioprosthetic heart valves.
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1540-8191.1992.tb00775.x
