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The Effects of Detergents on the Composition of Postsynaptic Densities (1984)

Somerville, Robert A. ; Merz, Patricia A. ; Carp, Richard I.

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 43 (1984), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: Abstract: A method of purifying postsynaptic densities (PSD) of Cohen et al. (1977) has been modified, primarily by the substitution of octyl glucoside as the detergent used to solubilize synaptosomal fractions. Subsequent extraction with other detergents resulted in the selective removal of specific polypeptides. In particular sulphobetaine 3–14 removed most of the β-tubulin but not α-tu- bulin. Sodium N - lauroyI sarcosinate completely destroyed the structural integrity of the PSD when the invitro formation of intermolecular disulphide bonds was minimized. These results suggest that the structure of PSDs is more labile than previously thought and demonstrate a technique for further examining their composition.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-4159.1984.tb06695.x

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