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A METHOD FOR DETERMINATION OF ACETYLCHOLINE BY SLOW PYROLYSIS COMBINED WITH MASS FRAGMENTOGRAPHY ON A PACKED CAPILLARY COLUMN (1979)

Polak, R. L. ; Molenaar, P. C.

Oxford, UK : Blackwell Publishing Ltd

Journal of neurochemistry 32 (1979), S. 0

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ISSN: 1471-4159

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: A new procedure is described for the estimation of ACh by pyrolysis-gas chromatography/ mass spectrometry.ACh iodide and the iodides of ACh-d16 or propionylcholine are slowly demethylated at 250°C by pyrolysis on the tip of a glass probe after insertion into the heated entrance of a glass tube leading to a packed capillary column. The volatile tertiary amines are then carried by helium to the column and trapped in its initial part which is kept at about 60°C. After 2–3 min the chromatography is started when the amines are released by heating this part to the ambient temperature in the oven (165°C).Peaks due to demethylated ACh and propionylcholine are well separated. The limit of detection is about 0.3pmol. After pyrolysis of mixtures of ACh and either ACh-d16 or propionylcholine the peak amplitudes are linearly related to the doses.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1471-4159.1979.tb00364.x

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