ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Histone and casein phosphoprotein-kinase activities were determined in rat brain soluble fraction at various stages of development.Cyclic AMP-independent or basal histone kinase activity increased, whereas cyclic AMP-dependent activity decreased in whole soluble fraction with the age. On the contrary, whole soluble cyclic AMP-dependent and -independent casein kinases activities did not show any difference during development.The percentage of activation by cyclic AMP of histone kinase activity and [3H] cyclic-AMP binding activity in the soluble fraction decreased markedly during development.By DEAE-cellulose chromatography the histone kinase was separated mainly into 4 peaks; the fourth peak was strongly stimulated by cyclic AMP. Stimulation by cyclic AMP was higher in the 4-day-old rat brains than in the 9- and 30-day-old. In the 9-day-old rats the ratio of cyclic AMP-dependent histone kinase in respect to the cyclic AMP-independent enzyme was higher than in 4- and 30-day-old rats.Casein kinase activities in the brains of 9- and 30-day-old rats were separated by DEAE-cellulose chromatography into three peaks of which the third one was stimulated by cyclic AMP. Little, if any, difference was observed for casein kinase during the development. These results suggest that brain histone and casein kinase are different enzymes:
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1975.tb11880.x
