Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Journal of neurochemistry
18 (1971), S. 0
ISSN:
1471-4159
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract— Glycogen was prepared from rapidly frozen rat brain by the usual techniques and found to contain considerable amounts of non-glycogen carbohydrate. The crude glycogen was partially purified by extraction with hot or cold water and reprecipitation. Enzymic estimation showed that the carbohydrate extracted into hot water contained only 50 per cent of glucose after hydrolysis; of the hot water insoluble material, namely some 30 per cent of the total carbohydrate present in the crude glycogen, less than half of the carbohydrate was released by hydrolysis in 1 M-HC1. The glycogen soluble in hot water incorporated 14C from [14C]glucose at considerably higher rates than the residual material and also decreased more rapidly during post-mortem autolysis. Glycogen extracted into cold water was of higher purity than that extracted by hot water; although the material behaved as glycogen during precipitation and re-extraction it contained only 75 per cent of its carbohydrate as glucose. Contaminants included fucose, galactose and hexuronic acid. The rates of metabolism of the partially purified glycogen are compared with published rates; it is suggested that the observed rates are inaccurate due to the impurities present in brain glycogen prepared by classical techniques.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1471-4159.1971.tb12035.x
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