ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
An accurate and precise method was developed for the determination of carrageenan in milk-based infant formulas. Following digestion of protein with papain, carrageenan was precipitated with Hyamine 1622, a quatemary ammonium salt. The precipitate was washed to remove lactose, dissolved in sulfuric acid and the carrageenan assayed by color development with the phenol-sulfuric reagent. Filtrations and washings were facilitated with Millipore filters. Carrageenan recovery of spiked-formulas averaged 99.67% for ready-to-feed products and 95.74% for concentrated products, which respectively contained 7.1% and 14.2% lactose. To obtain these accuracies the reference carrageenan must be identical to the carrageenan in the product.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1977.tb01263.x
