ISSN:
1750-3841
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
,
Process Engineering, Biotechnology, Nutrition Technology
Notes:
SUMMARY The muscles of stress-susceptible animals exhibit an extremely rapid rate of glycolysis when subjected to either excision or exsanguination anoxia. The adaptation of the whole-body perfusion technique to the intact animal makes it possible to study the influence of a wide variety of reagents while maintaining the structural integrity of the musculature. This paper outlines the perfusion technique as applied in this laboratory to adult pigs, and in addition describes a biopsy procedure which permits the use of one longissimus muscle as an untreated control for the other, thereby overcoming the problem of wide inter-animal variability. Results are presented of experiments designed to validate the perfusion and biopsy routines, and an account is given of preliminary investigations of the effects of added calcium and magnesium ions.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2621.1971.tb15125.x
