ISSN:
1365-2761
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Abstract. Two monoclonal antibody probes were produced against PK'X’ cells. The parasites were partially purified by filtration and centrifugation of kidney tissue from rainbow trout with proliferative kidney disease and used as antigen for immunization of mice. The resulting monoclonal antibodies reacted with PK'X’ cell antigens in enzyme-linked immunosorbent assay and immunohistochemistry tests. One antibody (Mab 12) appeared to be specific for PK'X’in kidney tissue, while the other (Mab 18) cross-reacted with host cell antigens in the kidney tubules. These probes are invaluable tools for the investigation of parasite surface antigens and life cycle studies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2761.1992.tb00683.x
