ISSN:
1365-2761
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
,
Medicine
Notes:
Abstract. A cell surface product (VS-P1) of Vibrio salmonicida has been purified from culture supernatant by a combination of extensive dialysis, filtration and centrifugation, as well as by salt precipitation and hydrophobic chromatography. SDS-PAGE analysis showed that the monomeric form of the antigen is a single polypeptide with an apparent molecular weight of 40000. Size exclusion HPLC of purified VS-P1 as well as VS-Pl-containing fish serum revealed, however, oligomeric forms in the range from 300000 to more than 700000 daltons. The antigen contained 6% carbohydrate and several isolectric forms were distinguishable when analysed on an analytical isoelectric focusing electrophoresis system. A ‘sandwich’ ELISA, utilizing polyclonal antibodies, was developed for screening sera from both healthy and moribund Atlantic salmon for the presence of the VS-P1 antigen.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-2761.1988.tb00540.x
