ISSN:
1745-4514
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Process Engineering, Biotechnology, Nutrition Technology
Notes:
Immunological techniques are widely used for the detection of enzymes in food systems and for elucidating protein structure and function. The generation of anti-bodies recognizing membrane-bound proteins is difficult due to their hydrophobic nature, and reduced antigenicity. This review summarizes strategies for the production and purification of polyclonal antibodies recognizing membrane proteins. Applications such as enzyme immunoprecipitation, use of site-specific anti-peptide. antibodies for determination of the topology of membrane-embedded proteins, immunocytochemistry, and expression vector cloning are described.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1745-4514.1996.tb00550.x
