ISSN:
1550-7408
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
SYNOPSIS. Antibodies to purified chloroplast preparations from Euglena gracilis var. bacillaris were prepared in rabbits. Success was achieved only after removal of lipids by forming acetone powders af chloroplasts. Ouchterlony agar-diffusion procedures showed that light-grown cells (containing chloroplasts) have at least 3 antigens reacting with these antibodies and not detectable in the dark-grown cells (which contained proplastids but no chloroplasts), The antigens uniquely associated with the chloroplast appear sequentially during the development of the proplastid into the chloroplast on light induction of the dark-grown cells. The first of these antigens appears at about 10 hr of development. Mutants of the parent strain showed varying numbers of antigens which were cor-related with their capacities to form plastid structures. Cells developing abnormal chloroplasts at low light intensities did not reveal any antigenic differences from cells developing normal chloroplasts. Various antigenic bands were also found which were common to all cell types studied. These can be ascribed either to non-chloroplast contaminants in the original preparation used to immunize the rabbits or to antigens which, although not in the chloroplasts, are cross-reacting with chloroplast materials. In the case of the dark-grown cells, it is likely that portions of the proplastids are cross-reacting with chloroplast materials.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1550-7408.1965.tb01853.x
