ISSN:
1600-051X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Abstract Delmopinol is a new surface active anti-plaque agent that has demonstrated a low antimicrobial effect in vitro. By use of a vitality staining technique, the antimicrobial effect on bacteria in plaque samples was tested after rinsing with delmopinol or chlorhexidine. 6 healthy male subjects volunteered to rinse for 4 days using a double-blind cross-over study design with a wash-out period between the rinsing regimens. No oral hygiene measures were allowed during the test periods and each test period started with a professional tooth cleaning procedure 2 days before the start of rinsing to allow for plaque formation. Rinsing was performed with 0.2% delmopinol hydrochloride or 0.2% chlorhexidine digluconate 2 × a day. Small samples of plaque were collected from the buccal surfaces of premolars and 1 st molars before the first rinse on day 1 and then before and 1, 2, 4, 7, and 24 h after the last rinse on the 4th day. The plaque samples were immediately stained with propidium iodide and fluoresceine diacetate to visualize dead and vital microorganisms respectively. The vitality of the microflora was evaluated using a fluorescence microscope. The baseline vitality values were 91% for chlorhexidine and 86% for delmopinol. At day 4, the plaque vitality for chlorhexidine was approximately 40% up to 4 h and 50% at 7 h and 60% at 24 h after the last rinse. Corresponding values for plaque vitality after delmopinol rinsing were between 70 and 80% on all sampling occasions. The differences in plaque vitality between delmopinol and chlorhexidine were statistically significant on all sampling occasions (p〈0.05) except for the baseline values. The low antimicrobial profile of delmopinol confirmed in vivo suggests a low risk for an ecological shift of the oral microflora due to cidal effects but also that delmopinol has other modes of action as an anti-plaque agent than an antimicrobial action.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-051X.1992.tb00653.x
