ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The production of tumour necrosis factor (TNF) and interleukin 1 (IL-1) by lipopolysaccharide-activated mononuclear cells from 39 healthy donors was studied in vitro by bioassay and ELISA. The donors were typed for HLA-A, -B, -C, -DR, and -DP antigens. There was no detectable production of TNFβ (lymphotoxin). The intracellular levels of bioactive TNFα were minimal or undetectable in all cases. Cells from HLA-DR2+ individuals secreted significantly lower amounts of TNFα than cells from HLA-DR2− donors [2 ng/ml (1.5–4.4) and 7.5 ng/ml (3.9–8.3) respectively (medians 25–75%); P〈0.01]. The difference disappeared if the cells were preactivated for 2 days with 1000 U/ml of recombinant gamma interferon (rIFN-γ). In some individuals, the TNFα response increased considerably after IFN-γ priming, in particular in those possessing the HLA-DR2 antigen. In contrast, there was no detectable difference in the production of IL-1β between the donors, and the IL-1β response decreased significantly after rIFN-γ priming in HLA-DR2+ individuals [2.3 ng/ml (1.1–8.4) versus 7.2 ng/ml (5–7.9); P〈0.05] and in HLA-DR2− individuals [3 ng/ml (1.1–5.3) versus 5.7 ng/ml (3.9–7.5); P〈0.01]. There was no correlation between the TNFα and IL-1 responses and any of the other HLA-DR, -DP, or -B antigens. There was a significant positive correlation between the levels of TNFα measured by ELISA and by cytotoxicity assay. However, the TNFα-containing supernatants from 9 out of 37 individuals appeared to contain inhibitor(s) of the biological activity of TNFα. The presence of inhibitor(s) was not associated with any HLA antigens.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1988.tb01492.x
