ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
in the present study the fine structures of Leu-7-Leu-15+ and Leu-7+-Leu-15 cell subpopulations were characterized by using an immunogold-immunoperoxidase double labelling in electron microscopy. The densities of Leu-15 antigenic sites on both E rosetting (Er) and non-adherent/non-E rosetting (non-A/non-Er) Leu-15 positive cell surfaces were also evaluated by using an immunogold analysis in electron microscopy. A majority of Leu-7+ cells co-expressed I he Leu-15 antigen and showed an ultrastructural pattern specific for mature natural killer (NK) cells, i.e. abundant cytoplasm with many organelles. numerous electron dense granules and irregular outline. On the other hand, a minority of Leu-7- ceils did not express the Leu-15 antigen and showed a clearly different ultrastructural feature in comparison to Leu-7+-Leu-15+ cells T hus, the presence of the Leu-15 antigen on Leu-7 + cell surface corresponds to ultrastructural features specific to differentiated NK cells and may represent an expression of Leu-7+cell differentiation. An alternative hypothesis may he that Leu-7+-Leu-15- and l.eu-7-Leu 15 cells represent distinct cell lineages within non-A/non-Er Leu-7+ cells. Finally, the results of the present study provide proof that Leu-7+ antigen is more frequently represented on non-A/non-Er Leu-L-15+ cells than on Er Leu-15+ cells.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1986.tb01961.x
