ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Human neutrophils activated by phorbol myristatc acelatc were cytoioxic lo ox erythrocyles. as determined by the 51Cr release method. Maximal cytolysis was obtained with a phorbol myristute acetate concentration of 5 ng/ml and with an etTcctor to target cell ratio of 1:4. An inlact neutrophil metabolic burst and production of oxygen-dcrived-frec radicals were essential lor the cytotnxic event, since neulrophils from patients with chronic granulo-malous disease failed to exhibit any ox erythrocyte lysis. The target cell destruction was completely prevented by catalase, was unaffected by supcroxidc dismutase. and was reduced approximately to one-third by azide and cyanide. These data suggest that, under our experimental conditions, the ox erythrocyte killing by phorbol myristatc acetate-activated neutrophils mainly depends on myelopcroxidasc and hydrogen peroxide.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1983.tb00772.x
