Electronic Resource
Oxford, UK
:
Blackwell Publishing Ltd
Scandinavian journal of immunology
17 (1983), S. 0
ISSN:
1365-3083
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
An anti-idiotypic (α Id) serum was induced against anti-tetanus toxoid (α TET)-reactivc F(ab')2 fragments of a single donor. After appropriate adsorption the serum was shown to react only with α TET F(ab')2 fragments and not with F(ab')2 fragments deplcted of α TET reactivity or anti-diphtheria toxoid (DIP) or anti-purified protein derivative (α PPD) F(ab')2 fragments of this donor. Inhibition studies with soluble TET indicated that the anti-idiotypic serum partly reacts with determinants close to or within the antigcnic binding site. When tested on a small panel of α TET F(ab')2 fragments derived from unrelated donors, one out of four donors tested showed cross-reactivity. When tested on α TET F(ab')2 fragments derived from related family members, the donor's father and two sisters were found to cross-react, whereas the mother and two other sisters did not. The observed cross-reactivity was independent of the litres of α TET antibodies in the serum. Furthermore, two-dimensional gel electrophoretic studies carried oui on α TET IgG of Id-positive or Id-negative family members indicated that the expression of idiotypic determinants is linked to a particular heavy chain. Immunofluorescent staining with α Id and cytofluoro-graphic analysis of donor T-lymphoblast proliferation in response to TET showed that the T cells did not express Id determinants found on his α TET antibodies.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1365-3083.1983.tb00781.x
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