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Detection of Antigen-specific Cellular Immune Response by the in Vitro Production of T-Cell Growth Factor (1982)

ILONEN, J. ; SALMI, A.

Oxford, UK : Blackwell Publishing Ltd

Scandinavian journal of immunology 15 (1982), S. 0

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ISSN: 1365-3083

Source: Blackwell Publishing Journal Backfiles 1879-2005

Topics: Medicine

Notes: The amount of T-cell growlh factor (TCGF) produced in vitro in antigen-stimulated lymphocyte cultures was measured by induction of growth in cell dependent on the factor. The method distinguishes eight herpes-simplex-seronegative subjects from ten seropositive ones, and these results correlated with those of the lymphocyte blast transformation (LBT) test. The LBT responses and TCGF amounts produced in cultures with purified tuberculin (PPD) were also in good correlation (r = 0.833, P〈0.001). Considerable-levels of the factor were already present 12 h after the beginning of the culture. The method offers rapid quantification of cell-mediated immunity to specific antigens.

Type of Medium: Electronic Resource

URL: http://dx.doi.org/10.1111/j.1365-3083.1982.tb00679.x

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