ISSN:
1398-9995
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Background: Mites of the genus Suidasia are commonly found in house dust and may play an allergenic role in exposed populations. However, the allergenic potential and clinical impact of this genus has not been well established. The main objective of this project was to evaluate the allergenic role of the mite Suidasia medanensis.Methods: An extract of S. medanensis was prepared and the allergen composition determined by immunoblot. Specific IgE antibody levels to S. medanensis and Blomia tropicalis were evaluated by radioallergosorbent (RAST) in the sera of 97 allergic asthmatic patients and 50 nonallergic subjects. Cross-reactivity between S. medanensis and the mite species B. tropicalis and Dermatophagoides farinae was investigated by RAST and immunoblot inhibitions.Results: Seventy-one asthmatic patients sera (73.2%) had positive IgE reactivity to S. medanensis; 14 allergens with molecular weights ranging from 7.5 to 105 kDa were detected. The most frequently detected had molecular weights of 30–31 (54.8%), 24.5 (42%), 21 (38.7%), 47 (35%) and 58 kDa (35.5%). Blomia tropicalis extract inhibited IgE binding to nine of these identified allergens. Four B. tropicalis allergens were inhibited by S. medanensis extract. RAST inhibition results demonstrated a high degree of inhibition by B. tropicalis (87.2%) and D. farinae (90.9%) than by S. medanensis (32%).Conclusions: Sensitization to S. medanensis is common in asthmatic allergy patients in Cartagena. An important degree of cross-reactivity was established between S. medanensis and B. tropicalis, and D. farinae.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1398-9995.2004.00636.x
