ISSN:
1600-0560
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Disruption of the cell-cycle regulation through over-expression or mutation of cyclins and cyclin-dependent kinases has been implicated in carcinogenesis. In order to determine whether keratoacanthoma (KA) is unique or a variant of squamous cell carcinoma (SCC) and whether expression of mitosis-related antigens are associated with KAs’tendency to regress, we compared the immunohistochemiral expression of mitotic cyclins (cyclins A and B) and their cyclin-dependent kinase p34cde2 in 21 KAs, 8 regressing KAs, and 28 conventional squamous cell carcinomas. KAs showed both overlap and significant differences in expression of these mitosis-related antigens compared to SCCs. Basal and parabasal pattern of expression of cyclins A and B significantly predominated in KAs in contrast to SCCs which exhibited diffuse pattern (cyclin A 86%/cyclin B 64% vs. 25%/36%, p〈0.01). However, no differences in the highest mean level of expression in‘hot spot’loci of cyclins A and B were identified comparing KAs to SCCs (19%/12% vs. 25%/13%, p〉0.05). For the cyclin-dependent kinase p34cde2, no differences in pattern, distribution or mean levels of expression were found. For cyclins A and B, regressing KA showed significantly more regional tumor labeling (88%/88% vs. 57%/33%, p=0.03) and a lower mean level of immunoreactivity (5%/4% vs. 19%/12%, p=0.001) compared to mature KAs. These findings indicate a role for mitotic cyclins in the evolution of both SCC and KA. The overlapping patterns of expression for these mitosis-related antigens suggest that KAs represent a variant of SCC that exhibit an overwhelming but not absolute tendency to involute.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1600-0560.1999.tb01863.x
