ISSN:
1574-6968
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Biology
Notes:
We optimized a previously established two-plasmid system for the identification of Staphylococcus aureus promoters that are recognized by the alternative transcription factor σB. The method allowed the identification of 18 S. aureusσB-dependent promoters, 12 of which are reported here for the first time to be σB-dependent. S1-nuclease mapping of the respective transcriptional start points revealed that all the promoters contained sequences exhibiting high similarity to the consensus sequence of Bacillus subtilisσB-dependent promoters. The promoters governed expression of genes encoding proteins proposed to be involved in various cellular functions, including the stress response genes and virulence-associated clfA gene for fibrinogen-binding clumping factor. Comparison of the nucleotide sequences upstream of the identified transcription start points identified a σB consensus promoter (GttTaa-N12–15-gGGTAt) that is highly homologous to that of σB of B. subtilis.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1016/S0378-1097(04)00063-1
