ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
The role of the EGF superfamily of growth factors and their receptors during wound healing continues to unfold. The aim of the study was to determine the effect of overexpression of erbB3 in wound healing and whether topical application of its various ligands enhances repair. Partial thickness porcine wounds were transfected with recombinant adenoviral particles containing erbB3 receptor gene or a vehicle β-galactosidase gene (Lac Z). Highly similar ligands: Epidermal growth factor (EGF), Epiregulin (EPR), Heparin-binding epidermal growth factor (HB-EGF), Heregulin (HER) were topically applied to wounds boosted with erbB3 receptors. Enhanced wound healing parameters were assessed 5 days after initial treatment for reepithelization, dermal depth, macrophage infiltration, proliferating cells, angiogenesis, and apoptotic cells. Treatment with EPR and HB-EGF promoted greater resurfacing of the epidermal layer than EGF, HER, erbB3 only or vehicle treated wounds. Neodermal formation as measured by dermal or granulation depth was significantly different in the EPR and HB-EGF treated wounds compared to EGF, HER, erbB3 only or vehicle. Numerous hallmarks of enhanced wound maturity (fewer infiltrating macrophages, significant reductions in neovascularization, fibroblastic proliferation) were noted in EPR- and HB-EGF-treated wounds transfected with erbB3 as compared to vehicle controls. Only erbB3 wounds treated with these particular ligands showed significant increases in apoptotic cells. Our results indicate that wounds treated with this erbB3 receptor form supplied with topical application of EPR and HB-EGF exhibit synergistic and accelerating reparative effects on porcine partial thickness wounds as compared to vehicle. These in vivo findings suggest the complexity and evidence of synergism between the erbB receptor and its various ligands on wound healing.(This research work was funded by the NIH GM40437.)
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2005.130215ap.x
