Electronic Resource
Oxford, UK; Malden, USA
:
Blackwell Science Ltd/Inc.
Wound repair and regeneration
12 (2004), S. 0
ISSN:
1524-475X
Source:
Blackwell Publishing Journal Backfiles 1879-2005
Topics:
Medicine
Notes:
Introduction: We hypothesize that interleukin-6 receptor (IL-6r) blockade with IL-6r antibody will increase matrix metalloproteinase-3 (MMP-3) production in hypertrophic scar fibroblasts.To test this hypothesis, MMP-3 protein levels were measured in fibroblasts cultured from normal skin and hypertrophic scar with and without IL-6 receptor blockade. Methods: Primary cell cultures of normal skin (NSF) and hypertrophic scar fibroblasts (HTS) were obtained from the same patient (n = 5) and seeded into 6 well plates (40,000 cells/cm2) in complete media. Cells were treated with and without IL-6 receptor antibody and IgG (10 ng/ml) for 6 hours. Protein concentrations of MMP-3 are determined in equal volume of supernatants by ELISA.Statistical analysis was by unpaired t-test(*P 〈 0.05). Results: Blocking IL-6 receptors with its antibody showed significant increase in MMP-3 protein concentration in HTS compared to NSF.〈table frame="bottom"〉〈tgroup cols="4" align="left"〉〈colspec colnum="1" colname="col1" align="left"/〉〈colspec colnum="2" colname="col2" align="left"/〉〈colspec colnum="3" colname="col3" align="left"/〉〈colspec colnum="4" colname="col4" align="left"/〉〈thead valign="bottom"〉〈row rowsep="1"〉MMP-3(ng/ml)MMP-3(ng/ml)〈tbody valign="top"〉NSF1.6 ± 0.13HTS0.7 ± 0.37NSF + IL-6r0.3 ± 0.15HTS + IL-6r2.3 ± 0.5*NSF + IgG0.26 ± 0.16HTS + IgG0.75 ± 0.14 Conclusion: IL-6 receptor mediated signaling is involved in the pathogenesis of hypertrophic scar formation and its blockade may reduce hypertrophic scar formation.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1111/j.1067-1927.2004.0abstracteu.x
| Library |
Location |
Call Number |
Volume/Issue/Year |
Availability |
