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Digitale Medien

Cloning of the Rhizopus niveus pyr4 gene and its use for the transformation of Rhizopus delemar (1995)

Horiuchi, H. ; Takaya, N. ; Yanai, K. ; [weitere]

Springer

Current genetics 27 (1995), S. 472-478

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Details

ISSN: 1432-0983

Schlagwort(e): Rhizopus niveus ; pyr4 ; Rhizopus delemar ; Transformation

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Abstract We have cloned a pyr4 gene encoding orotidine-5′-monophosphate decarboxylase of the filamentous fungus Rhizopus niveus. The pyr4 gene of R. nivens has an open reading frame composed of 265 amino-acid residues and has two putative introns. We have also isolated a pyr4 mutant of Rhizopus delemar from 5-fluoroorotic acid-resistant mutants and transformed it with the pyr4 gene of R. niveus as a selectable marker. Introduced DNA was integrated into the chromosome in a multiple tandem array. The mitotic stability of the introduced DNA was increased by a repeated sporulation process. The expression of the Escherichia coli β-glucuronidase gene in R. delemar was successfully obtained under the control of the pgk2 gene promoter of R. niveus by co-transformation with the pyr4 gene.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00311218

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