ISSN:
1524-475X
Quelle:
Blackwell Publishing Journal Backfiles 1879-2005
Thema:
Medizin
Notizen:
Tissue repair and wound healing are complex processes that involve inflammation, granulation, and tissue remodeling. Angiogenesis plays a central role in wound healing. Previously, we have shown that picroliv, a natural product obtained from the roots of Picrorhiza kurrooa upregulates the expression of vascular endothelial growth factor (VEGF) in human umbilical vein endothelial cells and insulin like growth factor (IGF) in rats during hypoxia (Cellular and Molecular Life Sciences 1999; 56: 348–55). In the present study, we have investigated the effect of Picroliv in an ex vivo rat aorta ring model of angiogenesis. Picroliv enhanced the sprouting and migration of endothelial cells. In this study, we have investigated the effect of picroliv in both unimpaired and dexamethasone (DX)-induced impaired cutaneous healing in a full thickness punch wound model in rats. The animals in the impaired healing group having been pretreated with DX. We studied the tissues by histology and morphometry. The data showed picroliv treatment resulted in improved reepithelialization, neovascularization, and migration of various cells such as endothelial, dermal myofibroblasts, and fibroblasts into the wound bed. Immunohistochemical localization showed an increased VEGF and alpha smooth muscle actin staining consistent with increased number of micro vessels in granulation tissue. These findings suggest that picroliv accelerated wound repair and thus could be developed as a therapeutic angiogenic agent for the wound healing.(This work was supported by a grant (5 R21 AT000517-02) from the NCCAM, National Institute of Health, Bethesda, MD.)
Materialart:
Digitale Medien
URL:
http://dx.doi.org/10.1111/j.1067-1927.2005.130215c.x
